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The world’s first hair-thin fluorescence microscope.
The NeuroDeep v1.0 laboratory setup applies advanced holographic techniques to resolve microscopic images through a hair-thin objective (⌀100μm). The endoscopic imaging probe can be inserted into living brain tissue to observe processes and structures at submicron level of detail. The NeuroDeep laboratory setup for microendoscopy is ideally suited for in-vivo fluorescence imaging of deep brain regions in a uniquely atraumatic manner and with minimal impact on the brain’s natural activity. The instrument is a powerful tool for neuroscience, biomedical and pharma laboratories.
1 | Maintain Natural Brain Functions
The endoscope probe is 100 microns in diameter, but stable enough to penetrate into deep brain regions.
2 | Observe Deep Structures
Thanks to innovative probe design, the tissue along the fibre insertion path can be imaged with high accuracy.
3 | Precise Navigation
As soon as a structure or process of interest is identified on the screen, it can be examined more closely.
4 | Great Detail
Structures and processes smaller than a micrometre can be resolved. Here, dendritic spines are clearly observable.
High Resolution / High Speed
In-vivo recording of fluorescently labelled neurons in Thy1-GFP line mouse up to amygdala level through a 110 μm single-fibre:
(A) NeuroDeep lowers the probe through a craniotomy, penetrating the animal model’s brain without bending. (B) On the way down, neuronal structures in the fibre facet’s field-of-view can be recorded in real-time. (C) High resolution—slow frame rate: Submicrometric features, such as dendritic spines, can be imaged. (D) Lower resolution – fast frame rate: A population of 11 neurons is recorded simultaneously at an imaging rate sufficient for neuronal activity monitoring studies. (E) An image of the entire length of the insertion path was reconstructed in post-processing.
Discover what has never been seen – with microendoscopy.
Real-time recording of active neuron structures during insertion of a NeuroDeep probe, visible on the user interface.
- Study in anaesthetised mouse model (Thy1-GFP line).
- Depth 5 mm – level of the amygdala
- Field of view: approx. 100×100 μm
Advantages for Researchers
Minimal tissue damage
The small diameter of the holographic endoscope reduces the negative impact on natural brain functions and shortens the recovery time after surgery.
Structural changes and activity of subcellular structures such as synapses, dendritic spines and axons are visualized in real time.
In vivo microscopy in the deepest brain regions becomes possible, which is currently only feasible ex vivo in postmortem brain slices.
Connectivity in Action
Continuous imaging on the way into the brain – recordings from a 100µm NeuroDeep objective, inserted into the brain of an anaesthetised mouse model with dynamic refocusing.
In-vivo imaging through single Fibres
Holographic endoscope technology is an impactful innovation from the field of Neurophotonics. It is based on years of rigorous research conducted at renowned institutes, and was validated for deep-brain imaging in multiple independent laboratories.
In medicine and neuroscience, a large endoscopic probe size can cause considerable limitations and complications, such as bleeding and damages to internal organs. The hair-thin endoscope addresses this issue by utilising the narrowest possible channel able to transfer image information – a single multimode optical fibre. Our approach uses computer-controlled holographic modulators and principles of digital holography. Thanks to this breakthrough innovation, the complex light propagation through the medium of the fibre can be characterised and tailored to deliver advanced microscopy at the end of the minuscule probe.
DeepEn provides laboratories with powerful tools to study the deepest regions of the brain. Our mission is to support researchers in discovering, developing and applying the tools for prevention, diagnosis, and treatment of brain disorders.
DeepEn is funded by the Federal Ministry for Economic Affairs and Climate Action and the European Social Fund as part of the EXIST program.